Spatial Variation of Phytochemical and Antioxidant Activities of Olive Mill Wastewater: A Chemometric Approach

Olive mill wastewater (OMW) was obtained during the extraction of olive oil. It is typified by an elevated concentration sugars, acids, proteins, polyphenols, and organic matter. This makes removal OMW problematic for all oil-producing countries. Due to their high in these wastewaters are a source danger environment. research aimed study spatial distribution effect terms geographical origin production oil on phenolic content antioxidant activity OMWs. A chemometric approach using principal component analysis (PCA) hierarchical cluster (HCA) utilized. Physico-chemical characterization OMWs performed evaluate pollutant load setting following parameters: pH, dry matter, conductivity, chemical oxygen demand. Quantitative compounds shows that extract samples had phenolics varying from 238.26 ± 5.67 534.16 3.83 mg GAE/g extract, flavonoids 179.89 1.64 421.47 3.42 QE/g tannins 101.66 0.65 216.28 3.41 CE/g extract. Antioxidant determined two testing systems: DPPH ABTS assay. The IC50 varied 0.30 0.08 1.93 0.34 µg/mL, while it between 2.04 0.16 6.11 0.25 µg/mL method. indicated methods strongly correlated. Furthermore, important correlations were shown one hand other activities (DPPH, ABTS).